14-3-3 theta antibody (pSer)

Catalog No. ABIN337475

The Rabbit Polyclonal anti-14-3-3 theta antibody has been validated for WB, ELISA, IHC (p) and IP. It is suitable to detect 14-3-3 theta in samples from Synthetic.

Quick Overview for 14-3-3 theta antibody (pSer) (ABIN337475)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Synthetic

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-14-3-3 theta Antibody

Binding Specificity: pSer

Specificity: Binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. Weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif, with sequences containing phospho-Ser surrounded by Phe at the +1 position, with Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding non-phosphorylated sequences or with other phospho- Thr/Ser/Tyr -containing motifs.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic phospho- (Ser) 14-3-3-binding-motif peptides.

Isotype: IgG

Application Details

Application Notes: ELISA (1:1000), IHC-P, IP (1:50), WB (1:1000)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 Binding Motif

Target Type: Amino Acid

Pathways: Apoptosis, Myometrial Relaxation and Contraction