AKT Substrate (pSer), (pThr) antibody

Catalog No. ABIN337521

This anti- antibody is a Rabbit Polyclonal antibody detecting in ELISA, WB, IHC (p) and IP. Suitable for .

Quick Overview for AKT Substrate (pSer), (pThr) antibody (ABIN337521)

Target: AKT Substrate

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details

Binding Specificity: pSer, pThr

Specificity: Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic phospho- Akt substrate peptides.

Isotype: IgG

Application Details

Application Notes: ELISA (1:1000), IHC-P, IP (1:20), WB (1:1000)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C/-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.

Target Details

Target: AKT Substrate

Target Type: Chemical