Ubiquitin antibody

Catalog No. ABIN340405

This Mouse Monoclonal antibody specifically detects Ubiquitin in WB, IHC, IF, IHC (p), IP and IHC (fro). It exhibits reactivity toward Human and Cow.

Quick Overview for Ubiquitin antibody (ABIN340405)

Target: Ubiquitin (Ub)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Ubiquitin Antibody

Specificity: Recognizes ubiquitin, both conjugated and unconjugated. Reactivity has been shown in human, bovine, chicken, Drosophila and C. elegans.

Purification: Purified

Immunogen: Purified bovine ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin (KLH).

Type of Immunogen: Hapten

Isotype: IgG1

Application Details

Application Notes: Approved: IF (1:10), IHC, IHC-Fr, IHC-P, IP, WB (1:10)

Usage: Can be used for immunoassays, Western Blot, Immunofluorescence and Immunoprecipitation. Furthermore the antibody is useful for Immunohistology after heat-induced retrieval on both frozen and paraffin embedded sections. Immunohistology: 1:10. Immunofluorescence: 1:10. Western Blot: 1:10. For Western Blot it is recommended to use lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF (  pH 7.5)) for 30-60 minutes at 4?C, followed by extensive PBS washing.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway