Histone 3 antibody (H3K36me3)

Catalog No. ABIN3434046

This anti-Histone 3 antibody is a Rabbit Polyclonal antibody detecting Histone 3 in WB, IF, ChIP, DB, ICC, ChIP-seq and CUT&RUN. Suitable for Human and Mouse. This Primary Antibody has been cited in 5+ publications.

Quick Overview for Histone 3 antibody (H3K36me3) (ABIN3434046)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K36me3

Purification: Protein A Chromatography

Immunogen: This Histone H3 trimethylLys36 antibody was raised against a peptide containing trimethylLys36 of human histone H3.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN3434046)

Powers, Parvanov, Baker, Walker, Petkov, Paigen: "The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo." in: PLoS genetics, Vol. 12, Issue 6, pp. e1006146, (2016) (PubMed).

Ho, Nateras, Yan, Park, Jensen, Borges, Lee, Champion, Tibes, Bryce, Carballido, Todd, Joseph, Wong, Parker, Stanton, Castle: "A Multidisciplinary Biospecimen Bank of Renal Cell Carcinomas Compatible with Discovery Platforms at Mayo Clinic, Scottsdale, Arizona." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132831, (2015) (PubMed).

Pham, Inoue, Vu, Nguyen, Nakayashiki, Ikeda, Nakayashiki: "MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis." in: PLoS genetics, Vol. 11, Issue 7, pp. e1005385, (2015) (PubMed).

Feng, Zhang, Shea, Creighton, Coarfa, Hilsenbeck, Lanz, He, Wang, Fu, Nardone, Song, Bradner, Mitsiades, Mitsiades, Osborne, Schiff, OMalley: "An epigenomic approach to therapy for tamoxifen-resistant breast cancer." in: Cell research, Vol. 24, Issue 7, pp. 809-19, (2014) (PubMed).

Mews, Zee, Liu, Donahue, Garcia, Berger: "Histone methylation has dynamics distinct from those of histone acetylation in cell cycle reentry from quiescence." in: Molecular and cellular biology, Vol. 34, Issue 21, pp. 3968-80, (2014) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Molecular Weight: 17 kDa

Gene ID: 3020