CD248 antibody (C-Term)

Catalog No. ABIN343918

This Rabbit Polyclonal antibody specifically detects CD248 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD248 antibody (C-Term) (ABIN343918)

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD248 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD248 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: At least two isoforms of TEM1 are known to exist, this antibody recognizes both isoforms.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD248

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Alternative Name: TEM1 / CD248

Background: Name/Gene ID: CD248

Synonyms: CD248, CD248 antigen, CD164 sialomucin-like 1, CD248 antigen, endosialin, CD248 Molecule, endosialin, Endosialin, TEM1, Tumor endothelial marker 1, CD164L1

Gene ID: 57124

UniProt: Q9HCU0