Hepatitis C Virus antibody (AA 1-61, AA 1-142)

Catalog No. ABIN344246

The Mouse Monoclonal anti-Hepatitis C Virus antibody has been validated for ELISA and WB. It is suitable to detect Hepatitis C Virus in samples from Hepatitis C Virus (HCV).

Quick Overview for Hepatitis C Virus antibody (AA 1-61, AA 1-142) (ABIN344246)

Target: Hepatitis C Virus (HCV)

Reactivity: Hepatitis C Virus (HCV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hepatitis C Virus antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Clone: A1-3D1

Product Details anti-Hepatitis C Virus Antibody

Binding Specificity: AA 1-61, AA 1-142

Specificity: Reactive with Recombinant Capsid Protein C and Envelop Protein M (core) (1-142aa) and Synthetic Capsid Protein C (1-61aa). Recognizes different antigenic determinants of HCV capsid protein. Does not cross-react with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).

Purification: Protein G purified

Immunogen: Synthetic peptides derived from HCV capsid protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:1), WB (0.5 - 1 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. ELISA: Indirect ELISA method. The testing plates were coated with HCV Non-Structural Protein (NS), Capsid Protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV C (OD492 value >2) and mixture of NS and C (OD492 value >1.5). Showed no reaction with HCV NS (OD492 value < 0.03). Western Blot: 0.5-1 μg/mL will allow visualization 0.1 μg/lane (Recombinant CPC+EPM (core)), 0.5 μg/lane (Synthetic CPC), and 0.1 μg/lane (Recombinant Chimeric HCV Polyprotein). Testing is under reducing and non-reducing conditions.

Comment:

Target Species of Antibody: Hepatitis C Virus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 40 % glycerol.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Hepatitis C Virus

Target: Hepatitis C Virus (HCV)

Alternative Name: HCV / Hepatitis C Virus

Target Type: Virus

Background: Name/Gene ID: HCV

Synonyms: HCV