COL2 antibody

Catalog No. ABIN344746

This Mouse Monoclonal antibody specifically detects COL2 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Chicken.

Quick Overview for COL2 antibody (ABIN344746)

Target: COL2 (Collagen, Type II (COL2))

Reactivity: Human, Mouse, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This COL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-COL2 Antibody

Specificity: Recognizes both alpha 1 (II) and alpha 3 (XI) chains which have identical primary structure. It also reacts with pepsin-digested type II collagen. Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Species cross-reactivity: Human, Chicken, Mouse, Salamander.

Purification: Purified

Immunogen: Purified type II collagen, obtained from adult chicken sternum by limited pepsinization.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (1:200), WB (1:200 - 1:400)

Usage: In Western blotting, antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. Western Blot of CNBr peptides of collagen II shows that this antibody reacts with CB11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. ELISA. Western Blot: 1:200-1:400. Cartilage is an appropriate positive control. Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1 mg/mL Tris HCl,  pH 2 for 15 minutes at RT or 15 minutes at 37?C.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Collagen Type II