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Histone 3 antibody (ciArg2, ciArg8, ciArg17)

H3 Reactivity: Human WB, IHC, ELISA, ICC Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN347088

Datasheet as PDF

Target See all Histone 3 (H3) Antibodies

Histone 3 (H3)
Binding Specificity
59

47

45

42

41

37

36

35

35

35

34

32

31

29

29

29

26

25

23

23

23

22

20

18

18

17

17

16

16

16

16

16

15

14

14

13

13

12

12

12

12

12

11

11

10

10

10

9

9

8
ciArg2, ciArg8, ciArg17
Reactivity
1400

941

861

59

42

41

41

40

39

35

35

30

29

22

19

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Human
Host
1218

243

8

4
Rabbit
Compatible Secondaries
Clonality
1121

351

1
Polyclonal
Conjugate
822

81

63

63

63

62

62

62

37

33

23

17

17

17

17

17

14

2

1
This Histone 3 antibody is un-conjugated
Application
1059

411

379

313

252

188

181

179

163

161

142

127

119

48

38

34

32

20

16

14

7

7

6

4

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Specificity

Reacts with human Histone H3 Citrulline at the 3 positions: 2, 8 and 17

Cross-Reactivity (Details)

Reacts with mono or di H3-Cit. Not tested in other species. May cross reacts with other species due to sequence homology.

Purification

Antiserum

Immunogen

Synthetic peptide derived from Nter domain of human Histone H3 containing Citrulline at position 2-8-17

Isotype

IgG

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H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC : 1/2 000 to 1/20 000 - WB : 1/5 000 to 1/50 000

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Must be reconstituted in distilled water.

Storage

4 °C/-20 °C

Storage Comment

Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date

24 months
Target

Histone 3 (H3)

Alternative Name

Histone H3 (H3 Products)

Synonyms

H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background

This core component of the nucleosome can be citrullinated on arginines 2, 8 and 17 by PADI4. These modifications blocks methylation and so represses the transcription

Gene ID

440093

UniProt

Q6NXT2