IL17F antibody

Catalog No. ABIN349631

Supplier Product No.: 209-401-b31

The Rabbit Polyclonal anti-IL17F antibody has been validated for WB and ELISA. It is suitable to detect IL17F in samples from Human.

Quick Overview for IL17F antibody (ABIN349631)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17F antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL17F Antibody

Supplier Product No.: 209-401-b31

Supplier: Rockland

Purpose: IL-17F Antibody

Cross-Reactivity (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-F in cell supernatants and certain body fluids.

Characteristics: Synonyms: rabbit anti-IL-17F antibody, rabbit anti-Interleukin-17F antibody, Cytokine ML-1, Interleukin-24

Purification: This purified antibody has been heated to 56°C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-F protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This purified antibody has been tested for use in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 15.0 kDa in size corresponding to human IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL17F

Background: Background: IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. Interleukin 1 receptor antagonist is secreted by various types of cells including immune, epithelial and adipocytes. Anti-IL-17F antibody is ideal for investigators involved in Cancer, Cardiovascular and Immunology research.

Gene ID: 112744

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity