SAE1 antibody (pSer185)

Catalog No. ABIN349635

Supplier Product No.: 600-401-b24

This Rabbit Polyclonal antibody specifically detects SAE1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SAE1 antibody (pSer185) (ABIN349635)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SAE1 Antibody

Binding Specificity: pSer185

Supplier Product No.: 600-401-b24

Supplier: Rockland

Purpose: SAE1 phospho S185 Antibody

Cross-Reactivity (Details): This purified antibody is directed against human SUMO Activating Enzyme E1 protein.

Characteristics: Synonyms: rabbit anti-SAE1 pS185 antibody, rabbit anti-SUMO activating enzyme subunit 1 pS185 antibody, Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, SUA1, SAE-1

Purification: The product was purified from monospecific antiserum by affinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to phosphorylated SAE1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific antibody reacts with human SAE1 pS185 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 2 μg/mL

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1

Background: Background: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.

Gene ID: 10055

NCBI Accession: NP_005491

UniProt: Q9UBE0