Catalase antibody

Catalog No. ABIN349684

This Rabbit Polyclonal antibody specifically detects Catalase in WB. It exhibits reactivity toward Zea mays, Wild Cabbage, Tomato, Spinach, Oryza sativa, Nicotiana tabacum, Nicotiana benthamiana, Barley and Arabidopsis thaliana and has been mentioned in 2+ publications.

Quick Overview for Catalase antibody (ABIN349684)

Target: Catalase (CAT)

Reactivity: Zea mays, Wild Cabbage, Tomato, Spinach, Oryza sativa, Nicotiana tabacum, Nicotiana benthamiana, Barley, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Catalase Antibody

Specificity: This antibody recognizes all three isoforms of Arabidopsis thaliana catalase.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 57 / 55kDa
In Chlamydomonas, HydA is present in low levels of 1 µg/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are published. to detect HydA1/A2 in Chlamydomonas extracts.The amount loaded per well corresponds to 2 µg of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 µg of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ngof recombinant HydA protein.

Purification: serum

Immunogen: KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At1g35090), catalase-3 (Q42547, At1g20620),

Application Details

Application Notes: Recommended Dilution: 1 : 8000 (ELISA), 1 : 2000 with standard ECL (WB).

Comment:

To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.1 % sodium azide is added as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Storage: -20 °C

References for anti-Catalase antibody (ABIN349684)

Marok, Tarrago, Ksas, Henri, Abrous-Belbachir, Havaux, Rey: "A drought-sensitive barley variety displays oxidative stress and strongly increased contents in low-molecular weight antioxidant compounds during water deficit compared to a tolerant variety." in: Journal of plant physiology, Vol. 170, Issue 7, pp. 633-45, (2013) (PubMed).

Ray, Mishra, Das, Sabat: "Expression and purification of soluble bio-active rice plant catalase-A from recombinant Escherichia coli." in: Journal of biotechnology, Vol. 157, Issue 1, pp. 12-9, (2012) (PubMed).

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: Cat

Background: AGI Code: At4g35090
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.

Molecular Weight: expected: 57 kDa, apparent: 55 kDa

UniProt: Q96528, P25819, Q42547

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism