NEFH antibody

Catalog No. ABIN349701

Cited in 1 publication. The Mouse Monoclonal anti-NEFH antibody (Clone NF-05) (ABIN349701) specifically detects NEFH in WB, ELISA and IHC (fro). The antibody is reactive with Human, Rat and Pig samples.

Quick Overview for NEFH antibody (ABIN349701)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: NF-05

Product Details anti-NEFH Antibody

Purpose: Anti-Neurofilament H Purified

No Cross-Reactivity: Mouse

Cross-Reactivity (Details): Human, Porcine, Rat

Characteristics: The antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules

Isotype: IgG1

Application Details

Application Notes: ELISA: Capture antibody.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-NEFH antibody (ABIN349701)

Porchet, Probst, Dráberová, Dráber, Riederer, Riederer: "Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex." in: Neuroreport, Vol. 14, Issue 7, pp. 929-33, (2003) (PubMed).

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament H

Background: Neurofilament heavy,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFH, NEFH, Neurofilament heavy chain, CMT2CC

Gene ID: 4744

UniProt: P12036