ATG7 antibody (C-Term)

Catalog No. ABIN349977

This anti-ATG7 antibody is a Sheep Polyclonal antibody detecting ATG7 in WB and IHC. Suitable for Human.

Quick Overview for ATG7 antibody (C-Term) (ABIN349977)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATG7 Antibody

Binding Specificity: C-Term

Purpose: Sheep antibody to ATG7

Specificity: Specific for ATG7.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from c-terminal region of human ATG7 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7

Background: This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2 GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex composed of ATG3 and ATG7 plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Widely expressed especially in kidney liver lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy