Choline Acetyltransferase antibody

Catalog No. ABIN349982

The Rabbit Polyclonal anti-Choline Acetyltransferase antibody has been validated for IHC. It is suitable to detect Choline Acetyltransferase in samples from Mouse.

Quick Overview for Choline Acetyltransferase antibody (ABIN349982)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-Choline Acetyltransferase Antibody

Purpose: Rabbit antibody to ChAT

Specificity: Specific for ChAT.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from mouse ChAT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Isotype: IgG

Application Details

Application Notes: IHC. A working concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term.

Expiry Date: 12 months

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: ChAT

Background: Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2), also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy negative tests for anti-AChR antibodies and abrupt episodic crises with increased weakness bulbar paralysis and apnea precipitated by undue exertion fever or excitement. Inheritance is autosomal recessive.

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development