Ubiquitin antibody (N-Term)

Catalog No. ABIN350073

This anti-Ubiquitin antibody is a Goat Polyclonal antibody detecting Ubiquitin in WB, IHC, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for Ubiquitin antibody (N-Term) (ABIN350073)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Ubiquitin Antibody

Binding Specificity: N-Term

Purpose: Goat antibody to Ubiquitin

Specificity: Specific for AUP1.

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic pentamer-peptide from the n-terminal region of human ancient ubiquitous protein 1 (AUP1) conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody works nicely in both paraffin,frozen sections. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.

UniProt: A1L134

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway