Caveolin-1 antibody (Cytoplasmic Domain)

Catalog No. ABIN350198

The Sheep Polyclonal anti-Caveolin-1 antibody has been validated for WB and IHC. It is suitable to detect Caveolin-1 in samples from Human.

Quick Overview for Caveolin-1 antibody (Cytoplasmic Domain) (ABIN350198)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Caveolin-1 Antibody

Binding Specificity: Cytoplasmic Domain

Purpose: Sheep antibody to CAV1

Specificity: Specific for Caveolin-1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the 1t cytoplasmic domain of human Caveolin-1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species including rat mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: CAV1

Background: FUNCTION: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. SUBCELLULAR LOCATION: Golgi apparatus membrane, Single-pass membrane protein. Cell membrane, Single-pass membrane protein. Note: Potential hairpin-like structure in the membrane. Membrane protein of protein of caveolae. TISSUE SPECIFICITY: In muscle and lung less so in liver brain and kidney.

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals