CHMP3 antibody (N-Term)

Catalog No. ABIN350226

Product Details anti-CHMP3 Antibody

Target: CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit antibody to VPS24

Specificity: Specific for CHMP3.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the n-terminal of human VPS24 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for CHMP3

Target: CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))

Alternative Name: VPS24 (CHMP3 Products)

Background: FUNCTION: Component of the ESCRT-III complex which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. Required for sorting/trafficking of EGF receptor. In case of infection the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins. SUBCELLULAR LOCATION: Cytoplasm cytosol. Membrane, Lipid-anchor. TISSUE SPECIFICITY: Widely expressed. Expressed in heart brain placenta lung liver skeletal muscle kidney and pancreas.

UniProt: Q9Y3E7