CHMP6 antibody (C-Term)

Catalog No. ABIN350233

Product Details anti-CHMP6 Antibody

Target: CHMP6 (Charged Multivesicular Body Protein 6 (CHMP6))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This CHMP6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Sheep antibody to CHMP6

Specificity: Specific for CHMP6.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the c-terminal region of mouse CHMP6 conjugated to an immunogenic carrier protein was used as the antigen. The peptide shares 95% identity with rat's.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for CHMP6

Target: CHMP6 (Charged Multivesicular Body Protein 6 (CHMP6))

Alternative Name: CHMP6 (CHMP6 Products)

Background: FUNCTION: Component of the ESCRT-III complex which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex it probably serves as an acceptor for ESCRT-II complex on endosomal membranes. SUBUNIT: Component of the endosomal sorting required for transport complex III (ESCRT-III) at least composed of CHMP1A CHMP1B CHMP2A CHMP2B CHMP3/VPS24 CHMP4A/SHAX2 CHMP4B/SHAX1 CHMP4C/SHAX3 CHMP5/SNF7DC2 and CHMP6/VPS20. In the complex it interacts directly with CHMP4A CHMP4B and CHMP4C. Interacts directly with SNF8 VPS25 and VPS36 components of the ESCRT-II complex. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Endosome membrane, Lipid-anchor . Note: Localizes to endosomal membranes.

UniProt: P0C0A3