HCN1 antibody (AA 400-450)

Catalog No. ABIN7698988

The Sheep Polyclonal anti-HCN1 antibody has been validated for WB and IHC. It is suitable to detect HCN1 in samples from Human.

Quick Overview for HCN1 antibody (AA 400-450) (ABIN7698988)

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This HCN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HCN1 Antibody

Binding Specificity: AA 400-450

Purpose: Sheep antibody to HCN1

Specificity: Specific for HCN1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 400-450 of human HCN1 conjugated to an immunogenic carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for HCN1

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Alternative Name: HCN1

Background: FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP and at 10-100 times higher concentrations also by cGMP. May mediate responses to sour stimuli. SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2. Interacts with KCNE2. SUBCELLULAR LOCATION: Membrane, Multi-pass membrane protein. TISSUE SPECIFICITY: Detected in brain in particular in amygdala and hippocampus while expression in caudate nucleus corpus callosum substantia nigra subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

UniProt: O60741

Pathways: Asymmetric Protein Localization