MAP1LC3A antibody

Catalog No. ABIN350462

Product Details anti-MAP1LC3A Antibody

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Sheep antibody to MAP1LC3A

Specificity: Specific for MAP1LC3A.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A MAP1A/MAP1B LC3 A) conjugated to ablue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50 μg,ml. The optimal workiing concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: MAP1LC3A (MAP1LC3A Products)

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. This protein is probably involved in formation of autophagosomal vacuoles. SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane, Lipid-anchor. Cytoplasmic vesicle autophagosome membrane, Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart brain liver skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

UniProt: Q9H492

Pathways: Autophagy