MAP1LC3A antibody

Catalog No. ABIN7206353

This Mouse Monoclonal antibody specifically detects MAP1LC3A in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MAP1LC3A antibody (ABIN7206353)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 5G10

Product Details anti-MAP1LC3A Antibody

Purpose: Mouse Anti-LC3A Mouse Monoclonal Antibody (5G10)

Specificity: LC3A protein detects endogenous levels of LC3A.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide of LC3A

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000,IHC 1:100-200,IF 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: LC3A

Background: Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alphaMAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by MAP1LC3A (microtubule associated protein 1 light chain 3 alpha) is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for MAP1LC3A. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Molecular Weight: 14,16kD

Gene ID: 84557

UniProt: Q9H492

Pathways: Autophagy