NSF antibody (N-Term)

Catalog No. ABIN350528

The Rabbit Polyclonal anti-NSF antibody has been validated for WB and IHC. It is suitable to detect NSF in samples from Human.

Quick Overview for NSF antibody (N-Term) (ABIN350528)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NSF Antibody

Binding Specificity: N-Term

Purpose: Rabbit antibody to NSF

Specificity: Specific for NSF.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from n-terminal of human NSF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and rat.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF

Background: FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. SUBUNIT: Homohexamer. Interacts with GABARAP and GABARAPL2. SUBCELLULAR LOCATION: Cytoplasm.

UniProt: P46459