VPS35 antibody (AA 190-240)

Catalog No. ABIN350530

This Rabbit Polyclonal antibody specifically detects VPS35 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for VPS35 antibody (AA 190-240) (ABIN350530)

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS35 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VPS35 Antibody

Binding Specificity: AA 190-240

Purpose: Rabbit antibody to VPS35 (190-240)

Specificity: Specific for VPS35.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 190-240 of human VPS35 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35

Background: FUNCTION: Essential component of the retromer complex a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). SUBCELLULAR LOCATION: Cytoplasm. Membrane, Peripheral membrane protein. TISSUE SPECIFICITY: Ubiquitous. Highly expressed in heart brain placenta skeletal muscle spleen thymus testis ovary small intestine kidney and colon.

UniProt: Q96QK1