Rho-related GTP-binding protein antibody

Catalog No. ABIN350723

Product Details anti-Rho-related GTP-binding protein Antibody

Target: Rho-related GTP-binding protein (RhO (pan))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Rho-related GTP-binding protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Sheep antibody to pan RhO

Specificity: Specific for RhO family.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA of 50-100 of human RhOA conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Alternative Name: pan RhO (RhO (pan) Products)

Background: The protein encoded by this gene is a small GTPase of the Rho-subfamily which regulates signaling pathways that control diverse cellular functions including cell morphology migration endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42 and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP) which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.