Rhodopsin antibody

Catalog No. ABIN350800

This Sheep Polyclonal antibody specifically detects Rhodopsin in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Rhodopsin antibody (ABIN350800)

Target: Rhodopsin (RHO)

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Rhodopsin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Rhodopsin Antibody

Purpose: Sheep antibody to Rhodopsin

Specificity: Specific for Rhodopsin.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human Rhodopsin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and rat.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Rhodopsin

Target: Rhodopsin (RHO)

Alternative Name: Rhodopsin

Target Type: Chemical

Background: FUNCTION: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. BIOPHYSICOCHEMICAL PROPERTIES: Absorption:Abs(max)=495 nm, SUBCELLULAR LOCATION: Membrane, Multi-pass membrane protein. TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.

UniProt: P08100

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction