RHOU antibody (Internal Region)

Catalog No. ABIN350806

Product Details anti-RHOU Antibody

Target: RHOU (Ras Homolog Gene Family, Member U (RHOU))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOU antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit antibody to RhoU

Specificity: Specific for RhoU.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the internal region of human Rho related GTP binding protein RhoU (CDC42 like GTPase 1 CDC42L1 Ryu GTPase) conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 90% identity with rat and mouse sequence.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for RHOU

Target: RHOU (Ras Homolog Gene Family, Member U (RHOU))

Alternative Name: RhoU (RHOU Products)

Background: Function: Acts upstream of PAK1 to regulate the actin cytoskeleton adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic side. Golgi apparatus membrane, Lipid-anchor. Cell junction ‰ focal adhesion. Cell projection ‰ podosome. Note: Localizes to podosomes in SRC-transformed cells. Tissue specificity: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach small intestine brain skeletal muscle and placenta.

UniProt: Q7L0Q8