RUNX1 antibody

Catalog No. ABIN350812

This Sheep Polyclonal antibody specifically detects RUNX1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for RUNX1 antibody (ABIN350812)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RUNX1 Antibody

Purpose: Sheep antibody to Runx1

Specificity: Specific for Runx1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human Runx1 conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: Runx1

Background: FUNCTION: CBF binds to the core site 5'-PYGPYGGT-3' of a number of enhancers and promoters including murine leukemia virus polyomavirus enhancer T-cell receptor enhancers LCK IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation. SUBUNIT: Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and THOC4. Interacts with ELF1 ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with MYST3 and MYST4. Interacts with SUV39H1 leading to abrogate the transactivating and DNA-binding properties of RUNX1. TISSUE SPECIFICITY: Highest levels in thymus bone marrow and peripheral blood.

UniProt: Q01196