SYT1 antibody (AA 10-60)

Catalog No. ABIN350924

The Rabbit Polyclonal anti-SYT1 antibody has been validated for WB and IHC. It is suitable to detect SYT1 in samples from Rat.

Quick Overview for SYT1 antibody (AA 10-60) (ABIN350924)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SYT1 Antibody

Binding Specificity: AA 10-60

Purpose: Rabbit antibody to Synaptotagmin 1 (10-60)

Specificity: Specific for SYTI.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 10-60 of rat Synaptotagmin 1 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin 1

Background: FUNCTION: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins syntaxin and AP2. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBCELLULAR LOCATION: Cytoplasmic vesicle secretory vesicle synaptic vesicle. Cytoplasmic vesicle secretory vesicle chromaffin granule. Note: Synaptic vesicles and chromaffin granules. TISSUE SPECIFICITY: Predominantly expressed in rostral phylogenetically younger brain regions and in some endocrine tissues.

UniProt: P21707

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport