VPS45 antibody

Catalog No. ABIN351329

The Rabbit Polyclonal anti-VPS45 antibody has been validated for WB and IHC. It is suitable to detect VPS45 in samples from Rat.

Quick Overview for VPS45 antibody (ABIN351329)

Target: VPS45 (Vacuolar Protein Sorting 45 (VPS45))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS45 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VPS45 Antibody

Purpose: Rabbit antibody to VPS45A

Specificity: Specific for VPS45.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from rat VPS45A conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous with the corresponding sequence in mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for VPS45

Target: VPS45 (Vacuolar Protein Sorting 45 (VPS45))

Alternative Name: VPS45A

Background: FUNCTION: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. SUBUNIT: Interacts with STX6 and ZFYVE20. SUBCELLULAR LOCATION: Golgi apparatus membrane, Peripheral membrane protein. Endosome membrane, Peripheral membrane protein. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network. TISSUE SPECIFICITY: Ubiquitous, expression was highest in testis and in brain. Detected in every part of the brain.

UniProt: O08700

Pathways: Transition Metal Ion Homeostasis, Synaptic Vesicle Exocytosis