ABHD4 antibody (AA 177-187)

Catalog No. ABIN351489

The Goat Polyclonal anti-ABHD4 antibody (ABIN351489) specifically detects ABHD4 in ELISA, IHC and IHC (p). The antibody is reactive with Human and Monkey samples.

Quick Overview for ABHD4 antibody (AA 177-187) (ABIN351489)

Target: ABHD4 (Abhydrolase Domain Containing 4 (ABHD4))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ABHD4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ABHD4 Antibody

Binding Specificity: AA 177-187

Specificity: Human ABHD4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Hamster, Panda, Dog, Bat, Horse, Pig (91%) Marmoset, Rat, Elephant, Rabbit, Opossum, Platypus (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RPTNPSEIRAP from an internal region of human ABHD4 (NP_071343.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Hamster, Panda, Dog, Bat, Horse, Pig (91%), Marmoset, Rat, Elephant, Rabbit, Opossum, Platypus (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:4000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ABHD4

Target: ABHD4 (Abhydrolase Domain Containing 4 (ABHD4))

Alternative Name: ABHD4

Background: Name/Gene ID: ABHD4

Synonyms: ABHD4, ABH4, Alpha/beta-hydrolase 4

Gene ID: 63874

NCBI Accession: NP_071343

UniProt: Q8TB40