KLKB1 antibody

Catalog No. ABIN351494

The Sheep Polyclonal anti-KLKB1 antibody has been validated for ELISA, IHC, IHC (p) and IEP. It is suitable to detect KLKB1 in samples from Human.

Quick Overview for KLKB1 antibody (ABIN351494)

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This KLKB1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectrophoresis (IEP)

Product Details anti-KLKB1 Antibody

Brand: IHC-plus™

Specificity: Specific for PK as demonstrated by immunoelectrophoresis and ELISA.

Purification: Ammonium sulfate precipitation

Immunogen: Human Prekallikrein (PK) purified from plasma.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IE, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.4, 0.15 M sodium chloride, 50 % glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KLKB1

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Alternative Name: KLKB1 / Plasma Kallikrein

Background: Name/Gene ID: KLKB1
Subfamily: Serine S1
Family: Protease

Synonyms: KLKB1, Fletcher factor deficiency, Fletcher factor, Kininogenin, Plasma kallikrein, PKK deficiency, Plasma kallikrein heavy chain, PPK, Prekallikrein deficiency, Plasma kallikrein light chain, Plasma prekallikrein, Kallikrein 3

Gene ID: 3818

UniProt: P03952

Pathways: Complement System, Brown Fat Cell Differentiation