Cathepsin L2 antibody

Catalog No. ABIN351497

The Mouse Monoclonal anti-Cathepsin L2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Cathepsin L2 in samples from Human.

Quick Overview for Cathepsin L2 antibody (ABIN351497)

Target: Cathepsin L2 (CTSL2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cathepsin L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BV55-1

Product Details anti-Cathepsin L2 Antibody

Brand: IHC-plus™

Specificity: The anti-Cathepsin V antibody recognizes human cathepsin V and procathepsin V. It does not cross-react with other cathepsins

Purification: Protein G purified

Immunogen: Synthetic peptide surrounding amino acid 21 of human cathepsin V.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 30 % glycerol, 0.5 % BSA, 0.01 % Thimerosal. Sourced in Ascites.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C,-80 °C

Storage Comment: Short term: -20°C
Long term: -70°C
Avoid freeze-thaw cycles.

Target Details for Cathepsin L2

Target: Cathepsin L2 (CTSL2)

Alternative Name: CTSV / Cathepsin V

Background: Name/Gene ID: CTSV
Subfamily: Cysteine C1
Family: Protease

Synonyms: CTSV, Cathepsin L2, Cathepsin L2, preproprotein, Cathepsin V, Cathepsin U, CTSU, CATL2, CTSL2

Gene ID: 1515

UniProt: O60911

Pathways: Toll-Like Receptors Cascades