STX2 antibody (AA 1-19)

Catalog No. ABIN351556

This Rabbit Polyclonal antibody specifically detects STX2 in WB, IHC, IP and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Pig, Dog, Monkey, Hamster, Drosophila melanogaster, Chicken, Sheep and Xenopus laevis.

Quick Overview for STX2 antibody (AA 1-19) (ABIN351556)

Target: STX2 (Syntaxin 2 (STX2))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Pig, Dog, Monkey, Hamster, Drosophila melanogaster, Chicken, Sheep, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STX2 Antibody

Binding Specificity: AA 1-19

Specificity: Recognizes rat Syntaxin 2 at ~35kD. Species cross-reactivity: human, mouse, bovine, canine, chicken, Drosophila, hamster, insect, monkey, porcine, rabbit, sheep, Xenopus.

Purification: Immunoaffinity purified

Immunogen: Rat syntaxin 2 synthetic peptide corresponding to amino acid residues 1-19 (KLH coupled).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot (Colorimetric): 1 μg/mL was sufficient for detection in 20 μg rat brain tissue extract. Positive control: HeLa Cell Lysate.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for STX2

Target: STX2 (Syntaxin 2 (STX2))

Alternative Name: STX2 / Syntaxin 2

Background: Name/Gene ID: STX2
Family: Syntaxin/epimorphin

Synonyms: STX2, Epimorphin, EPM, EPIM, STX2B, Syntaxin-2, Stx2A, STX2C, Syntaxin 2

Gene ID: 2054

UniProt: P32856