CD8 antibody (FITC)

Catalog No. ABIN356190

The Rat Monoclonal anti-CD8 antibody has been validated for FACS, IHC (fro) and IP. It is suitable to detect CD8 in samples from Mouse.

Quick Overview for CD8 antibody (FITC) (ABIN356190)

Target: CD8

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: 07-06-53

Product Details anti-CD8 Antibody (FITC)

Specificity: This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)

Characteristics: Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: < / = 2 μg/10^6 cells. (Ref.8,9)Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.10)Immunoprecipitation. (Ref.8)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3), and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 12525

UniProt: P01731