CD3 antibody

Catalog No. ABIN356220

The Rat Monoclonal anti-CD3 antibody (Clone C363-29B (YCD3-1)) (ABIN356220) specifically detects CD3 in FACS, IP, IHC (fro) and Func. The antibody is reactive with Mouse samples.

Quick Overview for CD3 antibody (ABIN356220)

Target: CD3

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: C363-29B (YCD3-1)

Product Details anti-CD3 Antibody

Specificity: This antibody is specific to CD3e (Mr. 25 kDa). The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naive T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)

Characteristics: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Purification: Purified

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (Ref.1-2)Immunoprecipitation. Functional Assay: In vitro Depletion of CD3+ cells. In vitro Activation of T cells (accessory cells must be provided). (Ref.1,3,4)Immunohistochemistry. (Ref.5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 12501

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway