Cytokeratin 1 antibody (pan)

Catalog No. ABIN356300

The Mouse Monoclonal anti-Cytokeratin 1 antibody has been validated for IHC (p), IF and IHC (fro). It is suitable to detect Cytokeratin 1 in samples from Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey and Pig.

Quick Overview for Cytokeratin 1 antibody (pan) (ABIN356300)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AE1-AE3

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: pan

Specificity: AE 1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors, reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins). This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. This antibody AE 1/AE 3 recognizes Low Molecular Weight Cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40) of the acidic family and CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5).
Cellular Localization: Cytoplasmic.Cellular Localization:

Cross-Reactivity (Details): Species reactivity (tested):Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.

Characteristics: Synonyms: pan Keratin, Cytokeratin Pan-reactive

Purification: Protein A Chromatography.

Immunogen: Human epidermal keratin. Remarks: Molecular weight of antigen: CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59), CK5 (58)CK6 (56) CK8 (52. 5). + CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) CK8 (52. 5).

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentrated antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. For FFPE tissue sections antigen retriever like trypsin is required. Recommended Positive Control: Human skin, lung ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Cytokeratin

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).Synonyms: Cytokeratin pan-reactive, pan Keratin