S100A1 antibody (FITC)

Catalog No. ABIN356321

This Mouse Monoclonal antibody specifically detects S100A1 in IF, FACS and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for S100A1 antibody (FITC) (ABIN356321)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A1 antibody is conjugated to FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: S100217

Product Details anti-S100A1 Antibody (FITC)

Specificity: This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis.
Cellular Localization: Cytoplasmic.

Cross-Reactivity (Details): Species reactivity (tested):Human, Monkey, Bovine, Rat and Mouse

Characteristics: Synonyms: S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain,S-100 protein beta chain, Astrocyte Marker, S-100 protein alpha subunit, S-100 proteinbeta subunit, S100 Alpha, S100 Beta

Purification: Protein A Chromatography.

Immunogen: Purified bovine S100 protein. Remarks: Molecular weight of antigen: ~22 kDa.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100A1

Background: S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100 % Soluble in ammonium sulfate at neutral pH . Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells.Synonyms: Astrocyte Marker, S-100 protein, S-100 protein alpha chain, S-100 protein alpha subunit, S-100 protein beta chain, S-100 protein beta subunit, S100 Alpha, S100 Beta, S100 Calcium binding protein, S100A, S100B

Gene ID: 528735

UniProt: P02639

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins