beta Actin antibody

Catalog No. ABIN356353

This Mouse Monoclonal antibody specifically detects beta Actin in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for beta Actin antibody (ABIN356353)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 4E8H3

Product Details anti-beta Actin Antibody

Specificity: This antibody is specific to Beta-Actin.

Characteristics: Synonyms: Loading Control, Actin cytoplasmic 1, Beta-Actin

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: ACTB recombinant protein is used to produce this monoclonal antibody.

Application Details

Application Notes: ELISA. Western blotting: 1/2000-1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actin beta / ACTB

Background: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Beta-actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.Synonyms: Actin cytoplasmic 1, Beta-Actin

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction