S100A1 antibody

Catalog No. ABIN356473

This Rabbit Polyclonal antibody specifically detects S100A1 in IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Cow and Monkey.

Quick Overview for S100A1 antibody (ABIN356473)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reactivity: Human, Mouse, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-S100A1 Antibody

Specificity: This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis. Cellular localization: Cytoplasmic.

Cross-Reactivity (Details): Species reactivity (expected):Monkey, Rat and Mouse.
Species reactivity (tested):Human, Bovine.

Purification: Protein A Chromatography

Immunogen: Purified S100 protein from Bovine.

Isotype: IgG

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. Immunofluorescence. Positive control: Melanoma or schwannoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 10 mM PO4, 150 mM NaCl, pH 7.4 containing 1 % BSA and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This antibody should not be diluted in Buffer which contains Detergent.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100A1

Background: S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100 % Soluble in ammonium sulfate at neutral pH . Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells.Synonyms: Astrocyte Marker, S-100 protein, S-100 protein alpha chain, S-100 protein alpha subunit, S-100 protein beta chain, S-100 protein beta subunit, S100 Alpha, S100 Beta, S100 Calcium binding protein, S100A, S100B

Gene ID: 6271

NCBI Accession: NP_006262

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins