UBE2I antibody (N-Term)

Catalog No. ABIN356594

Product Details anti-UBE2I Antibody

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific to UBE2I (N-term). Predicted to cross react with Mouse and DANRE (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2I.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBC9 / UBE2I (UBE2I Products)

Background: UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBalpha, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I

Gene ID: 7329, 9606

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway