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ISG15 antibody (C-Term)

This Rabbit Polyclonal antibody specifically detects ISG15 in IHC (p) and EIA. It exhibits reactivity toward Human.
Catalog No. ABIN356680

Quick Overview for ISG15 antibody (C-Term) (ABIN356680)

Target

See all ISG15 Antibodies
ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivity

  • 60
  • 7
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Host

  • 56
  • 7
  • 1
Rabbit

Clonality

  • 49
  • 15
Polyclonal

Conjugate

  • 44
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ISG15 antibody is un-conjugated

Application

  • 49
  • 35
  • 28
  • 9
  • 7
  • 7
  • 5
  • 2
  • 2
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 7
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    This antibody is specific to ISG15 (C-term).

    Purification

    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ISG15.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA: 1/1,000. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS containing 0.09 % (W/V) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

    Alternative Name

    ISG15 / G1P2

    Background

    ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.Synonyms: Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, UCRP, Ubiquitin cross-reactive protein

    Gene ID

    9636, 9606

    UniProt

    P05161
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