SETD8 antibody (C-Term)

Catalog No. ABIN356688

This anti-SETD8 antibody is a Rabbit Polyclonal antibody detecting SETD8 in WB and EIA. Suitable for Human.

Quick Overview for SETD8 antibody (C-Term) (ABIN356688)

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETD8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SETD8 Antibody

Binding Specificity: AA 364-393, C-Term

Specificity: This antibody is specific to Human SETD8 / SET07 (C-term)

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 364~393 amino acids from the C-terminal region of human SET07

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SETD8

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Alternative Name: SETD8 / SET07

Background: SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.Synonyms: H4-K20-HMTase SETD8, Histone-lysine N-methyltransferase SETD8, KMT5A, Lysine N-methyltransferase 5A, PR-Set7, PR/SET domain-containing protein 07, PRSET7, SET domain-containing protein 8, SET8

Gene ID: 387893, 9606

UniProt: Q9NQR1