Ubiquitin antibody (C-Term)

Catalog No. ABIN356747

The Rabbit Polyclonal anti-Ubiquitin antibody has been validated for WB, IF, IHC (p) and EIA. It is suitable to detect Ubiquitin in samples from Human.

Quick Overview for Ubiquitin antibody (C-Term) (ABIN356747)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Ubiquitin Antibody

Binding Specificity: AA 44-73, C-Term

Specificity: This antibody is specific to Ubiquitin (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 44~73 amino acids from the C-terminal region of Human Ubiquitin. Genename: UBC

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunofluorescence. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.Synonyms: Polyubiquitin-C, UBC

Molecular Weight: 8.565 kDa

Gene ID: 7316

NCBI Accession: NP_066289

UniProt: P0CG48

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway