PIAS4 antibody (N-Term)

Catalog No. ABIN356770

This Rabbit Polyclonal antibody specifically detects PIAS4 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for PIAS4 antibody (N-Term) (ABIN356770)

Target: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PIAS4 Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific to PIASy (N-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PIASy1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Reported to work in Immunoprecipitation (See Ref.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PIAS4

Target: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Alternative Name: PIAS4

Background: PIASy1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway, the wnt pathway and the steroid hormone signaling pathway. PIASy1 is involved in gene silencing, and promotes PARK7 sumoylation.Synonyms: E3 SUMO-protein ligase PIAS4, PIAS-gamma, PIASG, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma

Gene ID: 51588, 9606

UniProt: Q8N2W9

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Positive Regulation of Response to DNA Damage Stimulus