SPRED1 antibody (C-Term)

Catalog No. ABIN356945

The Rabbit Polyclonal anti-SPRED1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect SPRED1 in samples from Human.

Quick Overview for SPRED1 antibody (C-Term) (ABIN356945)

Target: SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRED1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SPRED1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects SPRED1 at C-term.

Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPRED1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for SPRED1

Target: SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))

Alternative Name: SPRED1

Background: SPRED1 is a tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.Synonyms: EVH1/Sprouty domain containing protein, NFLS, Spred-1, Suppressor of Ras/MAPK activation

Molecular Weight: 50477 Da

Gene ID: 161742, 9606

UniProt: Q7Z699

Pathways: Positive Regulation of Response to DNA Damage Stimulus