PYGM antibody (C-Term)

Catalog No. ABIN356948

The Rabbit Polyclonal anti-PYGM antibody has been validated for WB and EIA. It is suitable to detect PYGM in samples from Human. There is 1 publication available.

Quick Overview for PYGM antibody (C-Term) (ABIN356948)

Target: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYGM antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PYGM Antibody

Binding Specificity: C-Term

Specificity: This antibody detects PYGM at C-term.

Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PYGM.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-PYGM antibody (ABIN356948)

Hathout, Marathi, Rayavarapu, Zhang, Brown, Seol, Gordish-Dressman, Cirak, Bello, Nagaraju, Partridge, Hoffman, Takeda, Mah, Henricson, McDonald: "Discovery of serum protein biomarkers in the mdx mouse model and cross-species comparison to Duchenne muscular dystrophy patients." in: Human molecular genetics, Vol. 23, Issue 24, pp. 6458-69, (2015) (PubMed).

Target Details for PYGM

Target: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Alternative Name: Glycogen Phosphorylase Muscle Form

Background: PYGM catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5), also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.Synonyms: GPMM, Myophosphorylase, PYGM, Phosphorylase A, Phosphorylase B

Molecular Weight: 97092 Da

Gene ID: 5837, 9606

UniProt: P11217

Pathways: Cellular Glucan Metabolic Process