LIN28B antibody (Center)

Catalog No. ABIN357013

This Rabbit Polyclonal antibody specifically detects LIN28B in WB, FACS, IF, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for LIN28B antibody (Center) (ABIN357013)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-LIN28B Antibody

Binding Specificity: Center

Specificity: This antibody detects LIN28B at center.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide selected from the Center region of human LIN28B

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Alternative Name: LIN28B

Background: Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.Synonyms: CSDD2, LIN28B, Lin-28 homolog B

Molecular Weight: 26953 Da

Gene ID: 389421, 9606

UniProt: Q6ZN17