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TLR5 antibody (C-Term)

This Rabbit Polyclonal antibody specifically detects TLR5 in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN357052

Quick Overview for TLR5 antibody (C-Term) (ABIN357052)

Target

See all TLR5 Antibodies
TLR5 (Toll-Like Receptor 5 (TLR5))

Reactivity

  • 108
  • 57
  • 29
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Mouse

Host

  • 97
  • 20
  • 3
Rabbit

Clonality

  • 91
  • 29
Polyclonal

Conjugate

  • 61
  • 12
  • 12
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This TLR5 antibody is un-conjugated

Application

  • 95
  • 45
  • 44
  • 17
  • 13
  • 12
  • 12
  • 7
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 13
    • 8
    • 8
    • 8
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    This antibody detects TLR5 at C-term.

    Purification

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of mouse TLR5.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Target

    TLR5 (Toll-Like Receptor 5 (TLR5))

    Alternative Name

    TLR5

    Background

    TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. It also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

    Molecular Weight

    97626 Da

    Gene ID

    7100, 10090

    UniProt

    O60602

    Pathways

    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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