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Hippocalcin antibody (N-Term)

This Rabbit Polyclonal antibody specifically detects Hippocalcin in WB, IHC (p) and EIA. It exhibits reactivity toward Mouse and Human.
Catalog No. ABIN357112

Quick Overview for Hippocalcin antibody (N-Term) (ABIN357112)

Target

See all Hippocalcin (HPCA) Antibodies
Hippocalcin (HPCA)

Reactivity

  • 32
  • 22
  • 10
Mouse, Human

Host

  • 35
  • 2
Rabbit

Clonality

  • 35
  • 2
Polyclonal

Conjugate

  • 11
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Hippocalcin antibody is un-conjugated

Application

  • 28
  • 13
  • 13
  • 13
  • 9
  • 3
  • 3
  • 3
  • 2
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 15
    • 7
    • 2
    • 2
    • 1
    N-Term

    Specificity

    This antibody detects Hippocalcin at N-term.

    Purification

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Hippocalcin.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Target

    Hippocalcin (HPCA)

    Alternative Name

    HPCA / Hippocalcin

    Background

    Hippocalcin is a member of neuron-specific calcium-binding proteins family found in the retina and brain. This protein is associated with the plasma membrane. It has similarities to proteins located in the photoreceptor cells that regulate photosignal transduction in a calcium-sensitive manner. This protein displays recoverin activity and a calcium-dependent inhibition of rhodopsin kinase. It is identical to the rat and mouse hippocalcin proteins and thought to play an important role in neurons of the central nervous system in a number of species.Synonyms: BDR2, Calcium-binding protein BDR-2, Neuron-specific calcium-binding protein hippocalcin

    Molecular Weight

    22296 Da

    Gene ID

    3208

    NCBI Accession

    NP_002134

    UniProt

    P84074
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