GCAP1 antibody (C-Term)

Catalog No. ABIN357117

This anti-GCAP1 antibody is a Rabbit Polyclonal antibody detecting GCAP1 in WB, IHC (p) and EIA. Suitable for Human.

Quick Overview for GCAP1 antibody (C-Term) (ABIN357117)

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GCAP1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects GCAP1 at C-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GCAP1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for GCAP1

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Alternative Name: GCAP1 / GUCA1A

Background: Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90 % similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.Synonyms: C6orf131, GUCA1, Guanylate cyclase activator 1A, Guanylyl cyclase-activating protein 1

Molecular Weight: 22774 Da

Gene ID: 2978, 9606

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction